Beginning its tale in 19th century England, young aristocrat Jonathan Joestar finds himself locked in bitter rivalry with Dio Brando, a low-born boy who Jonathan's father took under his wing after the death of Dio's father. Discontent with his station in life, Dio's fathomless lust to reign over all eventually leads him to seek the supernatural powers of an ancient Aztec stone mask in the Joestar's possession—an artifact that will forever change the destiny of Dio and Jonathan for generations to come. Fifty years later, in 1938 New York City, Jonathan's grandson Joseph Joestar must take up his grandfather's mission and master the abilities necessary to destroy the stone mask and its immensely powerful creators who threaten humanity's very existence, the Pillar Men.

In a Japanese jail sits 17-year-old Jotaro Kujo: punk, fighter, delinquent...and possessed by a force beyond his control! Around the world, evil spirits are awakening: "Stands," monstrous invisible creatures which give their bearers incredible powers. To save his mother's life, Jotaro must tame his dark forces and travel around the world to Cairo, Egypt, where a hundred-year-old vampire thirsts for the blood of his family. But the road is long, and an army of evil Stand Users wait to kill JoJo and his friends...

The coastal town of Morioh is plagued by the "Bow and Arrow," an ancient artifact which manifests latent stand abilities in criminals and ordinary folk alike. At the same time as they are dealing with the huge influx of stand users—both good and bad—in Morioh, high schooler Josuke Higashikata and his friends are searching for the culprit of a series of homicides.

Naples, 2001. Giorno Giovanna is a small-time crook with one big dream—to become a "Gang-Star." No ordinary thief, Giorno has a connection to the remarkable Joestar bloodline, and possesses a Stand named Gold Experience. His dream starts to become reality when he meets Bruno Bucciarati, a mobster from the gang Passione and a fellow Stand user himself. Realizing that they share similar ideals, and both disagree with the gang's harmful affairs, Giorno reveals his goal to Bruno: with Bruno's help, he will reform Passione by overthrowing the boss. As Giorno becomes a member of Passione, and is inducted into Bruno's squad, he discovers that it is no simple gang; its numbers are teeming with Stand users. Now confronted by other squads of differing loyalties and unpredictable caliber, their goal to change the gang from the inside out will be a tough one. Taking on these adversaries, Giorno attempts to rise through the ranks and inch closer to the boss, a man who is shrouded in mystery.

Florida, U.S.A, 2011. After an accident while on a drive with her sweetheart, Jolyne Cujoh falls into a trap and is sentenced to fifteen years at the state-run maximum-security correctional facility Green Dolphin Street Prison—AKA "the Aquarium." On the verge of despair, she receives a pendant from her father that causes a mysterious power to awaken inside of her. "There are things in this world that are more terrifying than death, and what's happening in this prison is definitely one of them." A message from a mysterious boy who appears before Jolyne, inexplicable events that occur one after another, the horrifying truth told to her by her father when he comes to visit, and the name DIO…